25(OH)Vitamin D3-1-alpha-Hydroxylase


Catalogue number: HYD001

Product Type Primary Antibodies
Units100 µl
HostSheep
Application IHC
WB
Background
The polyclonal antibody is well suited for the detection of 25(Hydroxy)Vitamin D3-1-alpha-Hydroxylase in tissues and tissue extracts e.g. in HKC-8 cell line from human proximal tubules (1). The seco-steroid hormone 1,25-Dihydroxyvitamin D3 plays a vital role in Calcium metabolism, in tissue differentiation and during the growth of bone. The biosynthesis of the hormone is catalysed by a mitochondrial P450-enzyme, 25-Hydroxy-Vitamin D3 1-alpha-Hydroxylase. Although the enzyme has been detected in different tissues, the main site of Vitamin D3 synthesis is the kidney. 25(OH)Vitamin D3-1-alpha-Hydroxylase, 56 kDa Protein in Western Blot.

Positive Control
Kidney

Immunogen
Synthetic peptide from the coding sequence of murine 25(OH)-Vitamin-D3-1-alpha-Hydroxylase (aa 266-289)

Working Concentration
(liquid conc.) 1/500 (depending on fixation, species, unmasking method and secondary system)

Concentration
app. 7 mg/ml

Product
Sheep IgG fraction in glycine buffered NaCl solution pH 7.4; contains 0.1% sodium azide, 0.1% Epsilon-amino-n-caproic acid, 0.01% Benzamidine and 1 mM EDTA

Applications
WB, IHC

Cross Reactivity
Mouse, human

Storage
-20°C

References
1. Bland R, Walker EA, Hughes SV, Stewart PM, Hewison M. (1999) Constitutive expression of 25-hydroxyvitamin D3-1-alpha-hydroxylase in a transformed human proximal tubule cell line: Evidence for direct regulation of vitamin D metabolism by calcium. Endocrinology 140(5), 2027-2034. 2. Zehnder D, Bland R, Walker EA, Bradwell AR, Howie AJ, Hewison M, Stewart PM. (1999) Expression of 25-Hydroxyvitamin D3-1-alpha-hydoxylase in human kidney. J. Am. Soc. Nephrol. 10; 2465-2473 3. Bland R, Markovic D, Hills CE, Hughes SV, Chan SL, Squires PE, Hewison M (2004) Expression of 25-hydroxyvitamin D3-1alpha-hydroxylase in pancreatic islets. J Steroid Biochem Mol Biol. 89-90(1-5):121-125. 4. Takeyama K, Kitanaka S, Sato T, Kobori M, Yanagisawa J, Kato S. (1997) 25-Hydroxyvitamin D3 1alpha-hydroxylase and vitamin D synthesis. Science;277(5333):1827-30 (cloning of 1-alpha-Hydroxylase)

Caution
These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.