A431 cells stimulated with EGF

Catalogue number: X1003

Product Type Control Lysate
Units100 µl
Application Western Blot

Background
A431 cells are a positive control for EGFR as they express high levels of EGFR. They also used for cell cycle studies and can be stimulated with EGF to stimulate EGFR and are therefore a very useful model to study EGFR downstream signalling cascades eg shc, src, MAPK.

Synonyms: A431 stimulated with EGF control cell lysate

Product

Product Form: Unconjugated

Formulation: Provided as solution in Laemmli electrophoresis sample buffer. Samples need to be boiled for 3-5 minutes, before loading the gel.

Concentration: See vial for concentration

Applications
Suggested use: A volume of 10-25ul is suggested for immunobloting.

Functional Analysis: Western Blotting

Storage
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Product Stability: Reagents are stable for the period shown on the vial label when stored properly

Ship Conditions: Room Temperature

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.