ACTH, Corticotrophin

Catalogue number: AC100

Product Type Primary Antibodies
Units1 ml
Application IHC(P)
The antibody against ACTH is suitable for the detection of the hormone in tissue slices of the pituitary and in ectopic ACTH producing cells as it may occur in SCLC (small cell lung carcinoma). The adrenocorticotropic hormone ACTH is produced by cells of the pituitary gland as a pro-opiomelanocortin precursor. It is secreted into the blood as biological active ACTH (1-39). It is stimulating the synthesis and secretion of steroid hormones like cortisol and corticosterone from the adrenal cortex of mammalians and interrenal glands of other vertebrates. It is essential for the maintenance of glucose and salt in the blood. ACTH 1-24, no reactivity with CLIP (ACTH 17-39).

Positive Control
Pituitary, pituitary adenoma

No protease treatment required

Synacthen, ACTH (1-24)

Working Concentration
(liquid conc.) 1:50-1:100

Incubation Time
60 min at RT

Secondary Reagents
We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).

Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution/test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.


Cross Reactivity
Human, pig, rat


*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!