AFP, Alpha-Fetoprotein


Catalogue number: AF100

CloneA-013-01/F1-6P2A8-P2B9A9
IsotypeIgG1
Product Type Primary Antibodies
Units1 ml
HostMouse
Application IHC(C
P)
WB
Background
The antibody to alpha-fetoprotein may be used for the detection of AFP in fetal liver, primary liver carcinoma, and in metastases thereof. Most germ cell tumours are AFP-positive, too. Human alpha-fetoprotein (AFP) is synthesized in the embryonic yolk sac and during adult life in the liver. During childhood and youth only very low concentrations are found in blood, but it is found in high quantities in maternal and fetal blood during pregnancy. Exceptionally high concentrations have been found in different failures of fetal development. Tissue transformation as it occurs in primary hepatocarcinoma is accompanied by increased synthesis of alpha-fetoprotein, so that it can be used as an immunohistochemical marker of this disease. Human Alpha-Fetoprotein (no cross-reactivity with HSA, CEA, Ferritin, HCG, and PSA).

Positive Control
Fetal liver

Pre-Treatment
No protease pre-treatment required, but microwave treatment usually will intensify staining. We refer to BIOLOGO's Unmasking Fluid G (Art. No. DE007).

Immunogen
Purified Alpha-Fetoprotein from umbilical cord blood

Working Concentration
(liquid conc.) 1:10 to 1:50

Incubation Time
60 min at RT

Secondary Reagents
We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).

Product
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution/test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.

Applications
IHC(C, P), WB

Cross Reactivity
Human

Storage
2-8°C

References
1. MacDonald D.J., and Kelly A.M. (1978) The rapid quantitation of serum alpha-fetoprotein by two-site microenzyme immunoassay. Clin. Chim. Acta 87; 367-372. 2. Jacobsen G.K., Jacobsen M., and Clausen P.P. (1981) Distribution of tumor-associated antigens in the various histologic components of germ cell tumors of the testis. Am. J. Surg. Pathol. 5; 257-266.

Caution
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!