AGEs-BSA Antigen


Catalogue number: AGE01-P

Product Type Proteins & Peptides
Units1 mg
Application ELISA
IHC
WB
Background
Control protein and blocking protein for Western Blot, ELISA and IHC, blocks primarily AGE-1 antibodies. Under sterile conditions BSA was incubated with D-Glucose and 5 mM DTPA in 0,2 M phosphate buffer pH 7.4 at 37°C for 8 weeks. Low-molecular weight products and D-Glucose were eliminated by PD-10 column chromatography and dialyses against PBS pH 7.4.

Working Concentration
(liquid conc.) 1 ng/ml - 1 µg/ml

Concentration
1 mg/ml

Product
AGEs-BSA in PBS pH 7.4

Applications
ELISA, IHC, WB

Storage
-20°C

References
1. Takeuchi M. , Makita Z., Bucala R., Suzuki T., Koike T., and Kameda Y. (2000) Immunological evidence that non-carboxymethyllysine Advanced Glycation End-products are produced from short chain sugars and dicarbonyl compounds in vivo. Mol. Med. 6; 114-125. 2. Takeuchi M., Yanase Y., Matsuura N., Yamagishi S., Kameda Y., Bucala R., and Z. Matika (2001) Immunological detection of a novel Advanced Glycation End-Product. Mol. Med. 7; 783-791.

Caution
These reagents are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.