Amyloid A (mc1)

Catalogue number: AM505

Clonemc1
IsotypeIgG2a
Product Type Primary Antibodies
Units5 ml
HostMouse
Application Immunohistochemistry (frozen)
Immunohistochemistry (paraffin)

Background
Clone mc1 detects amyloid type AA. It may be used for the detection of protein deposits (which are not easily detected by HE staining), and will help distinguishing from amyloid types AL and B2M. The antibody may also be used for screening purposes in kidney and rectal biopsies. Amyloidosis describes a disease during which fibrillary proteins are secreted into the extracellular space of different organs. Histological stainings demonstrate eosinophilic deposits, which progressively displace surrounding parenchymatous tissues, resulting in the loss of organ function and eventually death. Chronic inflammation (type AA-amyloidosis) and plasma cell dyscrasia (type AL-amyloidosis) are the most frequent forms of amyloidosis occurring e.g. in kidney and rectal tissue. In neighbourhood of hemodialysis more frequently cases of amyloidosis of the B2M type have been reported. Human Amyloid Type AA.

Source

Immunogen: Human Serum-Amyloid A coupled to HRPO and Kininogen

Product
Cell culture supernatant containing 0.09% sodium azide

Purification Method: Cell culture supernatant containing 0.09% sodium azide

Concentration: S/N

Secondary Reagents: We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).

Specificity

Species Reactivity: Human

Applications
IHC(C, P)

Incubation Time: 60 min at RT

Working Concentration: (RTU) neat

Pre-Treatment: No protease pre-treatment required, but microwave treatment usually will intensify staining.

Positive Control: Kidney with amyloid deposits

Storage
2-8°C

Caution
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

References
1. Francis R.J. (1982) Amyloid. In: Theory and practice of histological techniques. Bancroft J.D. and Stevens A. eds. pp 155-179. 2. Linke R.P. (1984) Monoclonal antibodies against amyloid fibril protein AA. J. Histochem. Cytochem. 32(3); 322-328. 3. Donini U., Casanova S., Zucchelli P., et al. (1989) Immunoelectron microscopic classification of amyloid in renal biopsies. J. Histochem. Cytochem. 37(7); 1101-1106.

Protein Reference(s)

Database Name: UniProt

Accession number: P02735 (SAA_HUMAN)

Species Accession: Human