Anti-Rat IgG, biotinylated (Mouse absorbed)


Catalogue number: ZU124

Product Type Secondary Antibodies
Units1 ml
HostRabbit
Application ELISA
IHC(C
P)
WB
Background
The anti-IgG (H+L) has been affinity purified and biotin conjugated at a labelling rate of usually >98%. The binding capacity of the antibody predominatly has been preserved and remains in the range of 93-99% (as measured by ELISA). Biotinylated anti-immunoglobulins are preferred reagents used in immunohistochemistry, ELISA, Western Blot, and other techniques because of the exceptional high affinity of Biotin to Avidin and Streptavidin. cross-reactivities are batch dependent, please enquire!.

Immunogen
Affinity purified rat IgG

Working Concentration
(liquid conc.) dilute 1:50 up to 1:250

Concentration
250 µg/ml

Incubation Time
15-30 min

Secondary Reagents
We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art. No. AE005).

Product
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.

Applications
IHC(C, P), ELISA, WB

Cross Reactivity
Rat

Storage
2-8°C

References


Caution
These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!