bcl-2 Oncoprotein

Catalogue number: BCL002-0.5

Clone124
IsotypeIgG1, k
Product Type Primary Antibodies
Units0.5 ml
HostMouse
Application Immunohistochemistry (frozen)
Immunohistochemistry (paraffin)

Background
The bcl-2 antibody is suitable for the detection of this oncoprotein in normal and abnormal tissues. In normal lymphoidal tissue the antibody reacts with B lymphocytes in the mantle zone, whereas in the germinal centre very few cells are stained. In follicular lymphoma bcl-2 is over-expressed and the staining pattern may be used for differentiation from follicular hyperplasia, which are almost bcl-2 negative. Bcl-2 oncoprotein is a protein, which is localized on the inner membrane of mitochondria and on the endoplasmatic reticulum. It is an inhibitor of apoptosis. In the majority of follicular lymphoma translocation of the bcl-2 occurs, followed by deregulation of protein synthesis. Human bcl-2 oncoprotein .

Source

Immunogen: Synthetic peptide corresponding to human bcl-2 molecule aa 41-54 (GAAPAPGIFSSQPG-Cys)

Product
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.

Purification Method: Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.

Secondary Reagents: We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).

Specificity

Species Reactivity: Human, not mouse and rat

Applications
IHC(C, P)

Incubation Time: 60 min at RT

Working Concentration: (liquid conc.) 1:100-1:200

Pre-Treatment: Heat pre-treatment of formalin-fixed tissue with Unmasking Fluid C or G (Art. No. DE000 and DE007)

Positive Control: Tonsil

Storage
2-8°C

Caution
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

References
1. Tsujimoto Y. and Crone C.M. (1986) Analysis of the structure, transcripts, and protein products of bcl-2, the gene involved in human follicular lymphoma. Proc. Nat. Acad. Sci. (USA) 83; 5214-5218. 2. Hockenbery D., Nunez G., Milliman C., et al. (1990) Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death. Nature 348; 334-336. 3. Liu Y.J., Mason D.Y., Johnson G.D., et al. (1991) Germinal center cells express bcl-2 protein after activation by signals which prevent their entry into apoptosis. Europ. J. Immunol. 21; 1905-1910. 4. LeBrun D.P., Kamel O.W., Cleary M.L., et al. (1992) Follicular lymphomas of the gastrointestinal tract. Pathologic features in 31 cases and bcl-2 oncogenic protein expression. Am J. Pathol. 140; 1327-1335.

Protein Reference(s)

Database Name: UniProt

Accession number: P10415 (BCL2_HUMAN)

Species Accession: Human