CD15, human

Catalogue number: CD15-1

CloneMMA/LeuM1
IsotypeIgM
Product Type Primary Antibodies
Units1 ml
HostMouse
Application Flow Cytometry
Immunohistochemistry (paraffin)

Background
The antibody against CD15 CD15 is a helpful tool for the identification of Reed-Sternberg-Cells in Hodgkin's disease. Occasionally CD15 is exprimed also in large cell B- and T-cell lymphoma. CD15 or X-Hapten plays a role in mediating phagocytosis, bacterial activity and chemotaxis. It is present on >95% of granulocytes including neutrophils and eosinophils and to a lesser degree monocytes. It is also present on Reed-Sternberg cells and on some epithelia. Human CD15 (3-fucosyl-N-acetyllactosamine).

Source

Immunogen: U937 histiocytic cell line

Product
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.

Purification Method: Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.

Concentration: 50 µg/ml

Secondary Reagents: As secondary reagents we recommend the use of biotinylated anti-mouse IgM antibody (Art. No. BA-2020) in combination with streptavidin conjugates or ABC systems.

Specificity

Species Reactivity: Human

Applications
IHC(P), FACS

Incubation Time: 60 min at RT

Working Concentration: (liquid conc.) 1:15-1:25

Pre-Treatment: Formaldehyde-fixed material should be treated with Unmasking Solution C, citrate buffered solution pH 6 (Art. No. DE000) at 96-100°C followed by short term treatment with Pepsin (0.1%)

Positive Control: Reed-Sternberg cells in Hodgkin's disease

Storage
2-8°C

Caution
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

References
1. Skubitz K. et al. In: White Cell Differentiation Antigens. New York / Oxford University Press 1989; 800-805.