|Product Type||Primary Antibodies|
Clone O10 is well suited for labelling of CD1a in different tissues. It is used for the detection of Langerhans-Cells and dendritic cells in the skin and other tissues. Up to now CD1 has been identified as five different variants encoded by 5 different genes (they are designed as CD1a, b, c, d and e). CD1 is exprimed by cortical thymocytes, Langerhans cells in epithelia and dendric cells. Usually it doesn't occur in mature peripheral blood cells, but is exprimed intra-cytoplasmatically in activated T-cells. CD1a plays a role in the detection of graft versus host reaction, in the detection of interdigitating cells in artereosclerotic lesion and in asthmatic inflammation. Human CD1a, possible cross-reactivity with CD1b.
Immunogen: Human thymocytes
Protein G affinity purified IgG in PBS pH 7.3 with BSA and sodium azide (0.09%)
Purification Method: Protein G affinity purified IgG in PBS pH 7.3 with BSA and sodium azide (0.09%)
Concentration: 50 µg/ml
Secondary Reagents: We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art. No. AE005).
Species Reactivity: Human
Incubation Time: 60 min at RT
Working Concentration: (liquid conc.) 1-4 µg/ml
Pre-Treatment: Pre-treatment of formaldehyde-fixed material should be treated with Unmasking Solution E, TRIS-buffered EDTA pH 9 (Art. No. DE006) at 96-100°C
Positive Control: Skin, tonsil
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
1. Hunger RE, Sieling PA, Ochoa MT, Sugaya M, Burdick AE, Rea TH, Brennan PJ, Belisle JT, Blauvelt A, Porcelli SA, Modlin RL. Langerhans cells utilize CD1a and langerin to efficiently present nonpeptide antigens to T cells. J Clin Invest. 2004 Mar;113(5):701-8. 2. Takagi S, Miyagawa S, Ichikawa E, Soeda J, Miwa S, Miyagawa Y, Iijima S, Noike T, Kobayashi A, Kawasaki S. Dendritic cells, T-cell infiltration, and Grp94 expression in cholangiocellular carcinoma. Hum Pathol. 2004 Jul;35(7):881-6. 3. Poindexter NJ, Sahin A, Hunt KK, Grimm EA. Analysis of dendritic cells in tumor-free and tumor-containing sentinel lymph nodes from patients with breast cancer. Breast Cancer Res. 2004;6(4):R408-15. 4. Mazal PR, Hainfellner JA, Preiser J, Czech T, Simonitsch I, Radaszkiewicz T, Budka H. Langerhans cell histiocytosis of the hypothalamus: diagnostic value of immunohistochemistry. Clin Neuropathol. 1996 Mar-Apr;15(2):87-91. 5. Krenacs, L. et al. (1993) Immunohistochemical detection of CD1a antigen in formalin-fixed and paraffin-embedded tissue sections with monoclonal antibody O10. J. Pathol. 171: 99-104.