CD4, human T helper cells

Catalogue number: CD4-MEM-5

CloneMEM115
IsotypeIgG2a
Product Type Primary Antibodies
Units5 ml
HostMouse
Application Immunohistochemistry (frozen)
Immunohistochemistry (paraffin)

Background
The antibody is well suited for the detection of CD4+ cells in tissues and cell smears. Usually T-Cell lymphoma in the skin are CD4+ as well as HTLV-1 associated Leukaemia and lymphoma respectively. Clone MEM-115 blocks binding of HIV gp120 to CD4+ cells and inhibits also CD4-MHC Class II interactions. CD4 is a membrane glycoprotein with a single chain of app. 54 kDa MW. It is expressed by a subgroup of T-Cells (Helper/Inducer-Cells) which make app. 45% of peripheral blood lymphocytes. CD4 is also localized on about 80% thymocytes and to a low percentage on monocytes. It is involved in the detection of antigens, which are presented by antigen presenting cells. CD4 functions as a receptor for HIV (HTLV). Human CD4 molecule, D1 domain.

Source

Immunogen: Purified Antigen from Thymocyte and T-cell extract

Product
Cell culture medium with phosphate buffer, BSA and 0.09 % sodium azide**.

Purification Method: Cell culture medium with phosphate buffer, BSA and 0.09 % sodium azide**.

Secondary Reagents: We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).

Specificity

Species Reactivity: Human

Applications
IHC(C, P)

Incubation Time: 60 min at RT

Working Concentration: (RTU) neat

Pre-Treatment: The antigen epitope detected by clone MEM-115 may be sensitive to the treatment with H2O2. Therefore it is recommended to block endogenous peroxidases with 0.5% H2O2 in Methanol (10 min incubation). This step should be carried out prior to antigen unmasking! Antigen unmasking is performed at 96-100° Celsius in 1 mM EDTA pH 8.0 for 10-20 min.

Positive Control: Tonsil

Storage
2-8°C

Caution
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

References
1. Falini B, Stein H, Pileri S, Canino S, Farabbi R, Martelli MF, Grignani F, Fagioli M, Minelli O, Ciani C, et al. Expression of lymphoid-associated antigens on Hodgkin's and Reed-Sternberg cells of Hodgkin's disease. An immunocytochemical study on lymph node cytospins using monoclonal antibodies. Histopathology. 1987 Dec;11(12):1229-42. 2. Stein H, Spiegel H, Herbst H, Niedobitek G, Foss HD. [Lymphoid tissues and AIDS: role of lymphocytes and follicular dendritic cells(FDC)]Verh Dtsch Ges Pathol. 1991;75:4-19. Review. German. 3. Spiegel H, Herbst H, Niedobitek G, Foss HD, Stein H. Follicular dendritic cells are a major reservoir for human immunodeficiency virus type 1 in lymphoid tissues facilitating infection of CD4+ T-helper cells. Am J Pathol. 1992 Jan;140(1):15-22. 4. Daum S, Weiss D, Hummel M, Ullrich R, Heise W, Stein H, Riecken EO, Foss HD; Intestinal Lymphoma Study Group. Frequency of clonal intraepithelial T lymphocyte proliferations in enteropathy-type intestinal T cell lymphoma, coeliac disease, and refractory sprue. Gut. 2001 Dec;49(6):804-12.

Protein Reference(s)

Database Name: UniProt

Accession number: P01730 (CD4_HUMAN)

Species Accession: Human