CD45, LCA, human

Catalogue number: CD45-1

Clone2B11/PD7/26
IsotypeIgG1
Product Type Primary Antibodies
Units1 ml
HostMouse
Application Flow Cytometry
Immunohistochemistry (paraffin)

Background
The antibody pair labels B- and T-cell lymphoma, T- and B-cell leukaemia and may also be used for the characterization of malign lymphoma of the thyroid gland. Hairy cell leukaemia is also antibody positive, while myeloid (granulocytes) and erythroid tumours usually are negative. LCA, the human Leukocyte Common Antigen consists of a family of cell membrane glycoproteins with a molecular mass between 180 and 220 kDa. They are located on the surface of almost all leukocytes. The two clones included in the antibody cocktail have been characterized on the International Workshops on Human Leukocyte Typing III and IV and have been assigned as CD45 and CD45RB respectively Cell membrane of lymphoid cells (macrophages and polymorph nuclear lymphocytes stain weakly, while plasma cells usually are negative). No reactivity with non-hematopoetic cells, epithelia and connective tissue..

Source

Immunogen: Activated and neoblastic T-cells

Product
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.

Purification Method: Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.

Concentration: 30 µg/ml

Secondary Reagents: We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).

Specificity

Species Reactivity: Human

Applications
IHC(P), FACS

Incubation Time: 60 min at RT

Working Concentration: (liquid conc.) 1:10-1:50

Pre-Treatment: No pre-treatment with proteases necessary, improved staining with unmasking fluid G (Art. No. DE007)

Positive Control: Tonsil

Storage
2-8°C

Caution
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

References
1. Dalchau R., Kirkley J., and Fabre J.W. (1980) Monoclonal antibody to a human leukocyte-specific membrane glykoprotein probably homologous to the leukocyte-common (L-C) antigen of the rat. Europ. J. Immunol. 10; 737-744. 2. Streuli M., Morimoto C., Schrieber M., Schlossman S.F., and Saito H. (1988) Characterization of CD45 and CD45R monoclonal antibodies using transfected mouse cell lines that express individual human leukozyte common antigens. J. Immunology 141; 3910-3914 3. Pulido R., Cebrian M., Acevedo A., De Landazuri M.O., Sanchez-Madrid F. (1988) Comparative biochemical and tissue distribution study of four distinct CD45 antigen specificities. J. Immunol. 140; 3851-3857.