CD68 (KP1) Macrophages, human

Catalogue number: CD68KP1-0.5

CloneKP1
IsotypeIgG1, k
Product Type Primary Antibodies
Units0.5 ml
HostMouse
Application Immunohistochemistry (frozen)
Immunohistochemistry (paraffin)

Background
The antibody KP1 is well suited for the detection of tissue macrophages including Kupffer cells and spleen macrophages. CD68 is expressed on macrophages and monocytes. KP-1 is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. KP-1 reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Tumors of lymphoid origin are usually not stained. CD68 transmembran glycoprotein MW 110 kDa, localised in macrophages.

Source

Immunogen: Subcellular fraction of human alveolar macrophages

Product
Purified antibody with 0.2% BSA and 15 mM sodium azide as preservative

Purification Method: Purified antibody with 0.2% BSA and 15 mM sodium azide as preservative

Secondary Reagents: We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).

Specificity

Species Reactivity: Human, monkey, cat and rat. Does not react with pig, dog and chicken

Applications
IHC(C, P)

Incubation Time: 60 min at RT

Working Concentration: (liquid conc.) 1:100-1:200

Pre-Treatment: Unmasking with Pronase (Art. No. DE110)

Positive Control: Tonsil, spleen, lymph node

Storage
2-8°C

Caution
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

References
1. Fukuda M. (1991) Lysosomal membrane glycoproteins. Structure, biosynthesis, and intracellular trafficing. J. Biol. Chem. 266; 21327-21330. 2. Holness C.I. and Simmons D.L. (1993) Molecular cloning of CD68, a human macrophage marker related to lysosomal glycoproteins. Blood 81; 1607-1613. 3. Pulford KAF, Rigney EM, Micklem KJ, Jones M, Stross WP, Gatter KC, et al. (1989) KP1: a new monoclonal antibody that detects a monocyte/macrophage associated antigen in routinely processed tissue sections. J Clin Pathol;42:414-21. 4. Warnke RA, Pulford KAF, Pallesen G, Ralfkiaer E, Brown DC, Gatter KC, et al. (1989) Diagnosis of myelomonocytic and macrophage neoplasms in routinely processed tissue biopsies with monoclonal antibody KP1. Am J Pathol;135:1089-95.