Chicken anti Indole-3-butyric acid


Catalogue number: T0158p

ClonePolyclonal
Product Type Primary Antibodies
Units1 ml
HostChicken
Application ELISA
Immuno affinity chromatography
Background
Indole-3-butyric acid (IBA) is a plant hormone belonging to the group of auxins. It is used to stimulate root formation in plant cuttings.

Source
Chicken egg yolk

Product
Each vial contains 1 ml extract purified (PEG) chicken polyclonal antibody.

Applications
The antibody may be used in ELISA and Immuno-affinity-chromatography (IAC). Samples may include cutting medium samples and extraction of these samples may be performed using PBS-10% methanol, followed by neutralisation of the extract. Suggested dilution 1:12,800 in indirect ELISA (this titer is defined as the dilution that gives 50 % of the absorbance from the maximum absorbance when tested with ELISA). Plates are coated with 400 ng/ml ovalbumine-conjugated indole-3-butyric acid. HRP-conjugated anti-chicken IgY should be used as a tracer in a 1:8,000 dilution.

Cross Reactivity
Cross Reactivity Indole-3-aceteic acid < 0.1 %

Specificity
Immunogen: BSA-Indole-3-butyric acid conjugate, Target: Indole-3-butyric acid (IBA), CAS no.: 133-32-4, Solubility target: Methanol, ethanol.

Storage
Store at 4 °C or -20 °C. The working antibody solution is stable for at least 7 days at 4 °C. Precautions should be taken for storage for longer periods. Problems of longterm stability may occur with highly diluted solutions. No other preservative agent has been added to the present formulation. For long storage purposes in solution the addition of sodium azide to 0.02 % is advised with the appropriate precautions of use.

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.