Cytokeratin 8/18

Catalogue number: CK588

Clone5D3
IsotypeIgG2a
Product Type Primary Antibodies
Units5 ml
HostMouse
Application Immunohistochemistry (frozen)
Immunohistochemistry (paraffin)

Background
Clone 5D3 labels low molecular weight cytokeratins 8 and 18 in all simple epithelia, including glandular epithelia of the thyroid, breast, skin and epithelia of the gastrointestinal tract. It doesn't stain squamous epithelia and is used for the differentiation of carcinoma versus lymphoma. It will also detect micro-metastases in bone marrow. Cytokeratins are a group of water-insoluble filamentous proteins. They are constituents of the cytoskeleton of epidermal cells and other epithelial cells. Up to now 20 different cytokeratins have been characterised by gel electrophoresis. They have been subdivided into a basic and an acid subfamily. Most frequently the nomenclature according to Moll et al. (1982) is used. CK 8 is an acid CK, which is expressed by most epithelia except squamous epithelia. It is frequently expressed together with CK 18 (1,2). Cytokeratin 18 is expressed by simple epithelia of the intestinal tract, lung, bronchi, urinary tract, liver and gland ducts. Human intermediar filament cytokeratins 8 and 18 (MW 52,5 resp. 45 kDa).

Source

Immunogen: Cytokeratins isolated from human mamma carcinoma cell line MCF-7

Product
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002, if further dilution is required.

Purification Method: Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002, if further dilution is required.

Secondary Reagents: We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).

Specificity

Species Reactivity: Human, cat, lizard, sheep

Applications
IHC(C, P)

Incubation Time: 60 min at RT

Working Concentration: (RTU) neat

Pre-Treatment: In formaldehyde-fixed material pre-treatment with trypsin (0,1% in TBS containing 0,1% CaCl2, pH 7.8) 15 -30 min at 37°C is recommended.

Positive Control: Skin (sweat glands)

Storage
2-8°C

Caution
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

References
1. Moll R., Franke W.W., Schiller D.L., Geiger B., and Krepler R. (1982) The Catalog of Human Cytokeratins: Patterns of Expression in Normal Epithelia, Tumors and Cultured Cells. Cell 31; 11 ff. 2. Sun T.-T. Tseng S.C.G., Huang A.J.W., Cooper D., Lynch M.H., Weiss R., Eichner R., and Schermer (1985) Monoclonal antibody studies of keratin expression: A review. In: Intermediate Filaments , Wang E. et al. eds. N.Y. Acad. Sci. 455, pp 307 ff. 3. Angus B, Purvis J, Stock D, Westley BR, Samson AC, Routledge EG, Carpenter FH, Horne CH. (1987) NCL-5D3: a new monoclonal antibody recognizing low molecular weight cytokeratins effective for immunohistochemistry using fixed paraffin-embedded tissue. J Pathol. 153(4):377-384. 4. Martin de las Mulas J, Espinosa de los Monteros A, Bautista MJ, Gomez-Villamandos JC, Morales C. (1994) Immunohistochemical distribution pattern of intermediate filament proteins and muscle actin in feline and human mammary carcinomas. J. Comp. Pathol.111(4):365-381. 5. Méhes G, Witt A, Kubista E, Ambros PF. (2001) Circulating breast cancer cells are frequently apoptotic. Am. J. Pathol. 159(1):17-20.