Goat anti Mouse IgG1 IgG2a IgG2b IgG3 (Fc specific), conjugated with FITC
Catalogue number: GAM/IgG(Fc)/FITC
|Product Type ||Secondary Antibodies|
|Units||1 ml |
|Species reactivity ||Mouse|
The reactivity of the antiserum is directed to the Fc subunit of the IgG molecule. The antiserum contains antibodies to subclass-specific determinants as well as to determinants shared by two or more subclasses of IgG. In immunoelectrophoresis and double radial immunodiffusion, using various antiserum concentrations against normal mouse plasma, serum, and immunoglobulin fractions, no reaction is obtained with IgA, IgM and IgG/Fab fragments or any other serum protein. In immunocytochemical and immunohistochemical staining of IgG at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgG isotype in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Purified normal IgG, including all known subclasses, isolated from pooled mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
FITC-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C.
ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence.
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has been tested in double radial immunodiffusion anti several sera with the following results:
cow - Duck - Horse - Rat + Cat - Guinea Pig+ Monkey - Sheep - Chicken - Human - pigeon - Swine - Dog - Hamster ± Rabbit - Turkey -
Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal Goat antiSerum to Mouse IgG, Fc specific
The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Figure1: GAM/IgG(Fc)/FITC was used in a 1:100 dilution on swine colon, in combination with the primary monoclonal antibody to vimentin, RV202 (MUB1900P), used in a 1:1000 dilution. The green staining shows the connective tissue cells, while the blue color is DAPI staining for DNA (cell nuclei). As expected, no staining is seen in the epithelial cells of the intestinal crypts.
Figure2: GAM/IgG(Fc)/FITC was used in a 1:100 dilution on swine colon, in combination with the primary monoclonal antibody to vimentin, RV202 (MUB1900P), used in a 1:1000 dilution. The green staining shows the connective tissue cells, while the blue color is DAPI staining for DNA (cell nuclei). As expected, no staining is seen in the epithelial cells of the intestinal crypts.