Catalogue

Background
This antibody has been used for screening of HPV infections in various tissues. If positive staining turnes up, the analysis should be followed by testing for HPV 16 and 18 reactivity using antibody HP305 and/or specific PCR analysis. Human Papilloma virus (HPV) is responsible for the development of type and tissue specific papilloma in the oral cavity and in the larynx. In the skin papilloma virus can cause different types of warts and in the ano-genital region it is associated with condyloma or carcinoma. HPV types 1, 6 and 11 usually are associated with benign transformations of the tissues, while e.g. HPV types 16, 18 and 31 seem to be responsible for the development of some carcinoma like cervical carcinoma. The role of HPV in non-melanoic tumours of the skin is under discussion. Papillomavirus Type 1, 6, 11, 16, 18, 31.

Source

Immunogen: SDS-disrupted bovine papillomavirus type 1

Product
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 30 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002 if required.

Purification Method: Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 30 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002 if required.

Secondary Reagents: We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).

Applications
IHC(C, P)

Incubation Time: 60 min at RT

Working Concentration: (RTU) neat

Pre-Treatment: temperature antigen retrieval using 0.01 M citrate retrieval solution (pH 6.0) is recommended.

Positive Control: Infected cervical biopsy

Storage
2-8°C

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Cowsert et al. (1987) J. Natl. Cancer Inst. 79; 1053-1057. 2. Banks L.J. (1987) J. Gen. Virol. 68; 1351-1355. 3. Cason J., Patel D., Naylor J., et al. (1989) Identification of immunogenic regions of the major coat protein of human papillomavirus type 16 that contain type-restricted epitopes. J. General Virol. 70; 2973-2987. 4. Shepherd P.S., Lunny D., Brooks R., et al. (1992) The detection of human papilloma viruses in cervical biopsies by immunohistochemistry and in situ hybridization. Scand. J. Immunol. 36(11); 69-74.

Safety Datasheet(s) for this product: