Catalogue

Background
To sensitize cells in haemolytic complement assays and for screening of T and T receptor-bearing lymphocytes.

Source
Washed whole erythrocytes were prepared from fresh blood and used for intravenous immunization. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Product
Purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added. Homologous proteins of different species frequently share antigenic determinants. Cross-reactivity has not been tested. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water.

Formulation: Purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added. Homologous proteins of different species frequently share antigenic determinants. Cross-reactivity has not been tested.

Specificity
IgG fraction of polyclonal Rabbit antiSerum to Sheep erythrocytes.

Species Reactivity: Homologous proteins of different species frequently share antigenic determinants. Cross-reactivity has not been tested.

Applications
Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting. Haemolytic titre, at which 50% lysis occurs following mixing of equal volumes of antiserum, guinea pig complement (at a 1:60 dilution) and 5x108 RBC/ml, not less than 1:3,000 when tested with sheep erythrocytes.

Storage
The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.