Serotonin (5-HT)

Catalogue number: SE100

Product Type Primary Antibodies
Units1 ml
HostRabbit
Application Immunohistochemistry (frozen)
Immunohistochemistry (paraffin)

Background
The polyclonal antibody to serotonin is suitable for immunohistochemistry. It labels serotonin as well in normal cells and tissues as in carcinoids. Serotonin is a biogenic amine (5-Hydroxy-Tryptamine) which is produced in many eucaryontic cells. In Vertebrates it plays an important role as a neurotransmitter substance of the central and the peripheral nervous system. In the stomach and intestine it is produced by s.c. enterochromaffine cells in epithelia cell layers. In the blood it is mainly found in thrombocytes, basophilic granulocytes and in the granula of mast cells. Serotonin (5-Hydroxy-tryptamine).

Source

Immunogen: Hapten conjugated synthetic Serotonin (5-Hydroxy-tryptamine)

Product
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.

Purification Method: Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.

Secondary Reagents: We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art. No. AE005).

Specificity

Species Reactivity: Human and other spec.

Applications
IHC(C, P)

Incubation Time: 60 min at RT

Working Concentration: (liquid conc.) 1:10 - 1:50

Pre-Treatment: Pre-treatment with proteases is not required. Unmasking with unmasking fluid G (Code No. DE007) may be advantages.

Positive Control: Gastric and intestinal epithelia

Storage
2-8°C

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio​ accepts no liability for any inaccuracies or omissions in this information.

References
1. Veronica Estrada, Julia Krebbers, Christian Voss, Nicole Brazda, Heinrich Blazyca, Jennifer Illgen, Klaus Seide, Christian Jürgens, Jörg Müller, Rudolf Martini, Hoc Khiem Trieu & Hans Werner Müller
Low-pressure micro-mechanical re-adaptation device sustainably and effectively improves locomotor recovery from complete spinal cord injury
Communications Biology, Volume 1, Article number: 205 (2018)
https://doi.org/10.1038/s42003-018-0210-8


Safety Datasheet(s) for this product:
NM_Sodium Azide
/wp-content/uploads/SDS/Antibody SDS with Sodium Azide Noridic-MUbio.pdf