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Sheep anti Mouse 3BP2 (SH3 Binding Protein 2)

Catalog number: X1090P
Isotype IgG
Product Type Polyclonal Antibody
Units 250 µg
Host Sheep
Species Reactivity Mouse
Application Western Blotting

Background
3BP2 (SH3 binding protein 2) was identified as a protein binding to the SH3 domain of the Abl proto-oncogene. Murine 3BP2 cDNA encodes a 559 amino acid protein of 85 kDa consisting of an N-terminal PH domain, a C-terminal SH2 domain and intervening proline-rich sequences. 3BP2 interacts via its Sh2 domain with Syk family protein tyrosine kinases Syk, Zap70, Grb2, Cbl, PLC 1 and LAT in T lymphocytes and contributes to the transcriptional activation of NF-AT and AP-1. Mouse 3BP2 is 66% homologous to the human protein at the amino acid level.

Synonyms: Sh3bp2, SH3 domain-binding protein 2

Source

Immunogen: Antibody developed using SH2 domain of the 3BP2 protein fused to GST.

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Ammonium Sulfate Precipitation

Concentration: See vial for concentration

Applications
Detects 3BP2 protein by Western blot analysis. Detects a 85 kDa band in murine fibroblasts transfected with 3BP2. Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: Recombinant 3BP2 protein

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Decker, M., et al. Adaptor function for the Syk kinases-interacting protein 3BP2 in IL-2 gene activation. Immunity 1998, 9, 595-605 2. Songyang, Z., et al. Specific motifs recognized by the SH2 domains of Csk, 3BP2, fps/fes, GRB-2, HCP, SHC, Syk and Vav. Mol. Cell Biol. 1994, 14, 2777-2785

Protein Reference(s)

Database Name: UniProt

Accession Number: Q06649

Species Accession: Human

Safety Datasheet(s) for this product:

NM_Sodium Azide

SKU: X1090P Categories : Primary antibodies, Primary antibodies, Polyclonal Antibody
"Western blot analysis using anti-3BP2 at 10 µg/ml on recombinant full lenth 3BP2 protein (1), 3BP2 protein minus the PH domain (2), 3BP2 protein minus the PR domain (3) and 3BP2 protein minus the SH2 domain (4)." "Western blot analysis using anti-3BP2 at 10 µg/ml on recombinant full lenth 3BP2 protein (1), 3BP2 protein minus the PH domain (2), 3BP2 protein minus the PR domain (3) and 3BP2 protein minus the SH2 domain (4)."
"Western blot analysis using anti-3BP2 at 10 µg/ml on recombinant full lenth 3BP2 protein (1), 3BP2 protein minus the PH domain (2), 3BP2 protein minus the PR domain (3) and 3BP2 protein minus the SH2 domain (4)."

"Western blot analysis using anti-3BP2 at 10 µg/ml on recombinant full lenth 3BP2 protein (1), 3BP2 protein minus the PH domain (2), 3BP2 protein minus the PR domain (3) and 3BP2 protein minus the SH2 domain (4)."