Sheep anti Rat SSeCKS – myristylated (PKC) substrate

Catalogue number: S125P

IsotypeIgG
Product Type Polyclonal Antibody
Units250 µg
HostSheep
Species reactivity Rat
Application Immunohistochemistry
Immunoprecipitation
Western Blotting

Background
Activation of protein kinase C is a key signal transduction event in mesangial cell dedifferentiation and proliferation, yet little is known about downstream substrates or their roles in normal or diseased states. SSeCKS, a novel protein kinase C substrate originally isolated as a src-suppressed negative mitogenic regulator in fibroblasts, controls actinbased cytoskeletal architecture and scaffolds key signaling kinases such as protein kinase C and protein kinase A. Activation of protein kinase C is a key signal transduction event in mesangial cell dedifferentiation. A role for SSeCKS, a PKA/PKC scaffolding protein, has been implicated during the process of spermiogenesis and in the actin-based stellate morphology of mesangial cells

Synonyms: SSeCKS; A-kinase anchor protein 12; AKAP12; src-suppressed C kinase substrate

Source

Immunogen: Recombinant protein corresponding to amino acids 620-1596 of the SSeCKS protein.

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Ammonium Sulfate Precipitation

Concentration: See vial for concentration

Applications
Antibody can be used for Western blot (2-5 µg/ml), immunohistochemistry and immunoprecipitation. Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: Rat aortic smooth muscle

Storage
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Product Stability: Products are stable for one year from purchase when stored properly

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio​ accepts no liability for any inaccuracies or omissions in this information.

References
1. Lin, X., et al. 'A novel src- and ras-suppressed protein kinase C substrate with cytoskeletal architecture.' J. Biol. Chem. 1996, 271, 28430-28438 2. Siegel, S.M., et al. 'SSeCKS immunolabeling in rat primary sensory neurons.' Brain Res. 2002, 926, 126-136 3. Nelson, P.J., et al. 'Involvement of the protein kinase C substrate, SSeCKS, in the actin-based stellate morphology of mesangial cells.' J. Cell. Sci. 1999, 112, 361-370

Protein Reference(s)

Database Name: UniProt

Accession number: Q62766 (Rat)

Species Accession: Rat


Safety Datasheet(s) for this product:
NM_Sodium Azide
/wp-content/uploads/SDS/Antibody SDS with Sodium Azide Noridic-MUbio.pdf