Catalogue

Background
The reactivity of the antiserum is restricted to a subclass specific determinant on the pFc portion of the IgG4 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgG4 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgG4, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer’s choice have to be used. The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 250; in ELISA from 1:100 upwards; in Western blotting from 1:500 upwards.

Source
Highly purified monoclonal IgG4 isolated from pooled human serum.

Product
Purified monoclonal mouse IgG1 kappa conjugated with biotin, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration is 0.4 mg/ml. Biotin/IgG protein molar ratio (B/P) approximately 4.8. No foreign proteins added. Reconstitute the lyophilized product by adding 0.5 ml sterile distilled water.

Product Form: Biotin

Formulation: Purified monoclonal mouse IgG1 kappa conjugated with biotin, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration is 0.4 mg/ml

Specificity
Biotin-conjugated purified monoclonal Mouse antibody to Human IgG4, subclass specific. In an ELISA against human IgG subclasses, only IgG4 gave a strong positive reaction with the mouse anti-human IgG4 antibody. Reaction with human IgG1, IgG2 and IgG3 was minimal.

Species Reactivity: The antiSerum does not react with any other component of the Human Ig system or any other Human plasma protein as tested. This antiSerum has not been tested for cross-reactivity with other species.

Applications
ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.

Storage
The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4°C, not refrozen, and preferably used the same day.

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.