Catalogue

Background
Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament (NF) triplet proteins (70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble. All three NF proteins can be detected by immunohistochemical methods at day 9 or 10 after gestation in the Mouse embryo. Although IFPs of the neurofilament type are normally restricted to neurons, there are reports on their expression in non-neuronal cells as well. For example, in heart conduction myocytes NF proteins are expressed together with desmin. In tumorpathology ganglioneuroblastomas and some of the other neuroblastomas are strongly positive with the neurofilament antisera. Also, some neuro-endocrine malignancies may show NF positivity. In cell cultures of neural tissues the neurofilament antibodies can monitor in vitro differentiation.

Source
RNF402 is a Mouse monoclonal IgM antibody derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a neurofilament preparation of calf brain tissue.

Product
Each vial contains 0.1 ml monoclonal antibody in PBS containing 0.09% sodium azide. The antibody is purified by ammonium sulphate precipitation and will threfore contains fetal calf serum derived protein contaminants.

Formulation: Each vial contains 0.1 ml monoclonal antibody in PBS containing 0.09% sodium azide. The antibody is purified by ammonium sulphate precipitation and will threfore contains fetal calf serum derived protein contaminants

Purification Method: Ammonium sulphate precipitation.

Concentration: 1 mg/ml

Specificity
RNF402 reacts with both the phosphorylated and non-phosphorylated isoform of the 200 kD neurofilament protein.

Applications
RNF402 is suitable for immunoblotting, immunocytochemistry on permeabilised cells and immunohistochemistry on frozen and paraffin-embedded tissues. Optimal antibody dilution should be determined by titration; recommended range is 1:10 – 1:100 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:25 – 1:250 for immunoblotting applications.

Storage
The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Shipping Conditions: Ship at ambient temperature.

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Kuijpers, W., Tonnaer, E. L., Peters, T. A., and Ramaekers, F. C. (1991). Expression of intermediate filament proteins in the mature inner ear of the Rat and Guinea Pig, Hear Res 52, 133-46. 2. Bauwens, L. J., De Groot, J. C., Ramaekers, F. C., Veldman, J. E., and Huizing, E. H. (1992). Expression of intermediate filament proteins in the adult Human vestibular labyrinth, Ann Otol Rhinol Laryngol 101, 479-86.

Safety Datasheet(s) for this product: