Nordic-MUbio: Reagents for biomedical research

General information:

Nordic-MUbio offers over 2000 basic immunological reagents for biomedical and veterinary research, including antisera, antibodies, immunoconjugates, purified proteins, controls, serum protein standards and other calibrators. They  include highly specific monoclonal antibodies, as well as conjugated secondary immunoreagents for use in different test systems. Specifications, recommended working procedures and intended use are described in more detail in the product datasheets and on our website.

Product specificity groups:

Antisera to immunoglobulins (lg), lg subunits and structurally related polypeptides are raised against total Ig and specific lg subtypes purified from serum or appropriate secretions. Reagents intended for the detection of human antigens or antigens from other species, are cross adsorbed to meet the additional specificity criteria. Polyclonal antisera to subclasses of human Ig are raised against purified proteins and made specific by cross adsorption. They are analysed for specificity and titer in special immunodiffusion and immuno-electrophoresis tests. Antisera are also available for human and animal secretory proteins, components of the complement system, as well as coagulation factors and related proteins.

Monoclonal antibodies offered  by Nordic-MUbio include labelled and unlabelled monoclonal antibodies of murine and rat origin. Many of these highly specific, affinity purified immunoreagents have been published extensively. They are directed against immunoglobulin subtypes on the one hand, and cell and tissue specific molecules on the other. These reagents are widely used in biomedical and veterinary research, amongst others in immunology, virology, oncology, cardiovascular disease and neuropathology.The quality control of these reagents includes amongst others immunocytochemistry on cell lines, immunohistochemical staining of tissue sections and immunoblotting. Detailed product specificatons are available, and batch specific information will be provided with the reagents.

In addition to resarch reagents for in vitro studies on human and laboratory animal systems, specific antisera and immunoconjugates are available for use in studies of domestic and farm animal species.

Antibody titres:

The potency  or strength of an antiserum is commonly expressed as its titre, a figure referring to the end-point of an immunological test. Polyclonal antisera always react with different antigenic

determinants for which they have a variable affinity under different test conditions. Only part of the antibodies present will contribute to the test result under the given test conditions. Precipitating antibodies may not participate in non-precipitating antibody binding techniques and vice versa. Consequently, the titre of an antiserum is not a generally valid and constant characteristic. The titre of a cytochemical or immuno-assay grade reagent can be expressed as the optimum working dilution in a performance test. It can only serve as a general guideline since it varies with the test conditions.

Directions for handling and use:

Lyophilised Nordic-MUbio products may be stored at +4°C. The lyophilised content of the vial is reconstituted by adding sterile distilled water. Reconstituted antiserum should be frozen in aliquots at -20°C. When continuously used, the recontituted antiserum may be stored for about one week at +4°C.

After reconstitution, immunoconjugates and purified proteins are preferably divided into small aliquots, and stored at -20°C. Before using an aliquot, it is thawed slowly in the dark, spun down, and used to prepare working dilutions by adding sterile PBS (pH 7.2). Diluted conjugates should be stored at +4°C and not refrozen. They remain stable for at least 24 hours.

Non-lyophilised Nordic-MUbio monoclonal antibody reagents should be stored at 4°C, or in small aliquots at -20°C, but preferably not below -25°C due to the aggregation effect on the protein. Repeated thawing and freezing should be avoided.

The optimal working dilution of antisera, monoclonal antibodies and conjugates should always be established by titration. When used in fluorescence microscopy or in the fluorescence activated cell sorter (FACS), a working dilution between 1:40 and 1: 200 may serve as a general guideline. Likewise, biotin- and peroxidase-conjugates may be diluted for histochemical use between 1:500 and 1:2,000. When used in ELISA or Western blotting, working dilutions of the reagents may show a larger variation, i.e. between 1:1,000 and 1: 10,000 depending on the test conditions.

Caution and Warning:

Immunological products and all associated materials should be handled by qualified personel and appropriate precautions should be taken in their handling and disposal. They are intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

The products may contain sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water.

The product datasheets are as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

Nordic-MUbio, Rangeerweg 5A, 6114 BC Susteren, The Netherlands

Phone: +31 (0)6 83611669 –  E-mail: info@nordicmubio.com – www.nordicmubio.com