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Anti-Mouse/Rabbit IgG, biotinylated

Catalogue number: ZU114

Product Type Secondary Antibodies
Units1 ml
HostHorse
Application ELISA
Immunohistochemistry (frozen)
Immunohistochemistry (paraffin)
Western Blotting

Background
The anti-IgG (H+L) has been affinity purified and biotin conjugated at a labelling rate of usually >98%. The binding capacity of the antibody predominatly has been preserved and remains in the range of 93-99% (as measured by ELISA). Biotinylated anti-immunoglobulins are preferred reagents used in immunohistochemistry, ELISA, Western Blot, and other techniques because of the exceptional high affinity of Biotin to Avidin and Streptavidin. cross-reactivities are batch dependent, please enquire!.

Source

Immunogen: Affinity purified IgG from mouse and rabbit

Product
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.

Purification Method: Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.

Concentration: 700 µg/ml

Secondary Reagents: We recommend the use of BIOLOGO's Streptavidin-HRPO (Art. No. ZU054) and Streptavidin-AP (Art. No. ZU051).

Specificity

Species Reactivity: Mouse, rabbit (rat)

Applications
IHC(C, P), ELISA, WB

Incubation Time: 15-30 min

Working Concentration: (liquid conc.) dilute 1:50 up to 1:250

Storage
2-8°C

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio​ accepts no liability for any inaccuracies or omissions in this information.

References


Safety Datasheet(s) for this product:
NM_Sodium Azide
/wp-content/uploads/SDS/Antibody SDS with Sodium Azide Noridic-MUbio.pdf