Chicken anti Human fibronectin (N-terminal 29 kDa fragment) (not affinity purified)
Catalog number: AHF| Product Type |
Primary antibodies |
| Units | 10 mg |
| Host | Chicken |
| Application |
ELISA Western Blotting |
Source
A chicken was immunized with the N-terminal 29 kDa fragment of human fibronectin. After multiple immunizations, eggs were collected and IgY was purified from the egg yolk.
Product
Product Form: Egg yolk-derived purified IgY
Formulation: Phosphate buffered saline‚ pH 7.2 with 0.05% sodium azide
Purification Method: Egg yolk-derived purified IgY (non affinity purified)
Concentration: See vial for concentration.
Applications
For ELISA, recommended dilution: 1:10,000. Diluted 1:100, this antibody completely inhibits binding of Staphylococcus aureus to immobilized fibronectin N-terminal domain (coating concentration: 20 micrograms/ml).
Storage
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles.
Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.
References
1. Roche FM, Downer R, Keane F, Speziale P, Park PW, Foster TJ. 2004, The N-terminal A domain of fibronectin-binding proteins A and B promotes adhesion of Staphylococcus aureus to elastin. J Biol Chem. 279(37):38433-40.
Safety Datasheet(s) for this product:
NM_Sodium Azide