Double stranded RNA control (dsRNA 142 bp)Catalog number: 10080100
Our defined 142 bp long dsRNA sequence is intended to be used as a positive control in double-stranded (ds)RNA detection. The sequence does not contain any viral or potentially transforming elements. Positive control for dsRNA detection by Anti-dsRNA monoclonal J2, K1 und K2 antibodies.
Synonyms: Double stranded RNA control (dsRNA 142 bp)
In vitro synthesized RNA (heteropolymer consisting of unmodified ribonucleotides)
Lyophilized from a 1 mg/ml solution in STE Buffer (0.1 M NaCl, 1 mM EDTA, 50 mM Tris-HCl, pH 7.0). Solubility: To prepare a 1 μg/μl solution add 50 μl bidestilled, sterile water that has been treated with DEPC to remove RNase.
Purity: Absorbance spectrum and gel electrophoresis
Double stranded RNA control (dsRNA 142 bp) is suitable for use as a size standard in dsRNA and RNAi analysis on both non-denaturing polyacrylamide and agarose gels. We suggest to use it as a homogenous, well-characterized positive control for different dsRNA detection methods, e.g. for ELISA and dot-blots.
Functional Analysis: Sandwich-ELISA (relative activity to reference antigen)
Store at -20 °C to -80 °C upon reconstitution, avoid freeze/thaw cycles, store in aliquots.
Product Stability: Shelf Life: 12 months
Shipping Conditions: Ship on blue ice.
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.