Mouse anti Chromogranin ACatalog number: MUB0334S
Neurons and neuroendocrine cells contain membrane-delimited pools of peptide hormones, biogenic amines, and neurotransmitters with a characteristic electron-dense appearance. These vesicles, which are present throughout the neuroendocrine system and in a variety of neurons, store and release chromogranins and secretogranins, a unique group of acidic, soluble secretory proteins. Chromogranin A, a protein of 439 amino acids which is encoded on chromosome 14, and an apparent molecular weight of 68kDa, is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, phenochromocytomas, paragangliomas, and other neuroendocrine tumors, including small cell lung carcinoma.
LK2H10 is a Mouse monoclonal IgG1 antibody derived by fusion of NS-1 Mouse myeloma cells with spleen cells from a BABL/c Mouse immunized with an immunogen that has been isolated from Human pheochromocytoma.
Each vial contains 1 ml of culture supernatant of monoclonal antibody containing 0.09% sodium azide.
Formulation: Each vial contains 1 ml of culture supernatant of monoclonal antibody containing 0.09% sodium azide.
LK2H10 reacts with the soluble secretory protein Chromogranin A.
LK2H10 is useful for immunohistochemistry on paraffin embedded and frozen tissues. Optimal antibody dilution should be determined by titration; recommended dilution 1:10 when applied in the Indirect immunofluorescence method. With the ABC method the dilution can be 5-10x higher. Dilute immediately before use with PBS or TRIS-HCl pH 7,0.
The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.
1. Taupenot, L., Harper, K.L., O’Connor, D.T. (2003). The chromogranin-secretogranin family. N Engl J Med 348, 1134-49. 2. Lloyd, R.V. and Wilson, B.S. (1983). Specific endocrine tissue marker defined by a monoclonal antibody. Science 222, 628-30. 3. Wilson, B.S. and Lloyd, R.V. (1984). Detection of chromagranin in neuroendocrine cells with a monoclonal antibody. Am J Pathol 115, 458-68.
Database Name: UniProt
Accession Number: P10645
Safety Datasheet(s) for this product: