Mouse anti Human CD3 FITC - CD4 PE - CD8 Cy5-PECatalog number: X1046S
Three-Color Reagents (FITC/RPE/Cy5RPE)
The CD3 epitope is expressed on the epsilon chain of the CD3/T cell antigen receptor (TcR) complex. CD3 is present on 65-85% of thymocytes and has a mitogenic effect on peripheral blood T cells. CD3 indentifies human T cells expressing the 22-28,000 M.W. surface antigen. CD4 identifies human helper/inducer T cells expressing the 60,000 M. W. surface antigen (HLA class II reactive). CD4 is present in low density on monocytes. Identification of CD8 on human cytotoxic/ suppressor T cells expressing the 32 and 43,000 M.W. surface antigens.
Immunogen: CD4=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human perherial blood T lymphocytes. CD3=Derived from the hybridization of mouse NS-1 myeloma celss with spleen cells from BALB/c mice immunized with human thymocytes. CD8=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human perherial blood T lymphocytes.
Provided in phosphate buffered saline with 0.09% sodium azide
Product Form: Conjugated
Purification Method: Affinity Chromatography
Concentration: Titered for flow cytometry
PBMC: Add 15 µl of 3-ColorTM antibody reagent/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2° to 8° C. Wash twice with PBS. Wash twice with PBS or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 15 µl of Exalpha Biological's 3-ColorTM antibody reagent MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20° C. Lyse the whole blood. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Functional Analysis: Flow Cytometry Staining
Product should be stored at 4-8ºC. DO NOT FREEZE
Product Stability: See expiration date on vial
Shipping Conditions: Ship at ambent temperature
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.
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Database Name: UniProt
Accession Number: P07766, P01730, P01732
Species Accession: Human
Safety Datasheet(s) for this product: