Mouse anti Human Proximal Nephrogenic Renal Antigen, cln PN-15 (66.4.C2)
Catalog number: X1234M| Clone | 66.4.C2 |
| Isotype | IgG2b |
| Product Type |
Monoclonal Antibody |
| Units | 100 µg |
| Host | Mouse |
| Species Reactivity |
Human |
| Application |
Immunohistochemistry (frozen) Immunohistochemistry (paraffin) |
Background
This antibody reacts with a glycoprotein of 200 kDa present in proximal renal tubules. The antigen is a carbohydrate in nature and retained in formalin fixed paraffin embedded tissues. Other normal tissues that display the antigen include breast, parathyroid glands and epididymis. Among renal carcinomas, 93% of primary and 84% of metastatic carcinomas are positive for this antigen. Relatively few other tumor types are positive: breast cancers, teratocarcinomas and parathyroid adenomas.
Source
Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with renal cortical tissue extract and mouse myeloma cells
Product
Product Form: Unconjugated
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
Purification Method: Protein A/G Chromatography
Concentration: See vial for concentration
Applications
This antibody can be used for the differential diagnosis of carcinomas by Western blotting and immunohistochemistry on frozen and paraffin-embedded tissues. Protease digestion prior to antibody incubation enhances staining of tissue sections. Optimal concentration should be evaluated by serial dilutions.
Functional Analysis: Paraffin section immunostaining
Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Product Stability: See expiration date on vial
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.
References
1. Yoshida, S.O. and Imam, A., 'Monoclonal antibody to a proximal nephrogenic renal antigen: immunohistochemical analysis of formalin-fixed, paraffin-embedded human renal cell carcinomas.' Cancer Res. 1989, 49, 1802-1809.
2. Piron, A., et al. 'In vitro demonstration of a mitogenic activity in renal tissue extracts during regenerative hyperplasia.' Am. J. Physiol. 1998, 274, F348-F357.
Safety Datasheet(s) for this product:
| NM_Sodium Azide |