Catalogue

Background
The defined antibody specificity is restricted to beta-thromboglobulin as tested at the level of sensitivity of immunoprecipitation techniques. A single precipitin line is obtained in biddimensional electrophoresis against normal plasma and platelet concentrate lysate, which shows a reaction of identity with precipitated purified beta-thromboglobulin. The antiserum does not react with any other protein component of human serum or plasma. In plasma the beta-thromboglobulin can only be assayed in more sensitive methods as ELISA, because of the low concentration of the antigen: in electroimmunodiffusion (Laurell), immunonephelometry, solid phase assays and as catching or detection antibody in ELISA. Measurements of beta-thromboglobulin is of significance for the diagnosis of platelet disorders involving platelet alpha granules and to demonstrate increased platelet secretions in vivo.

Source
Beta-thromboglobulin is a platelet specific protein. In normal plasma mean concentrations of 30 ng/ml have been found. Highly purified beta-thromboglobulin has been isolated from platelets and is used for the immunization. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Product
Delipidated, heat inactivated lyophilized whole serum. Sodium azide 1 mg/ml. Total protein and IgG concentrations in the antiserum are comparable to those of normal pooled rabbit serum. No foreign proteins added.

Formulation: Delipidated, heat inactivated lyophilized whole serum. Sodium azide 1 mg/ml. Total protein and IgG concentrations in the antiserum are comparable to those of normal pooled rabbit serum. No foreign proteins added.

Specificity
Precipitating polyclonal Rabbit antiSerum to Human beta-thromboglobulin.

Species Reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins and their fragments, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.

Applications
Precipitation assays. In electroimmunodiffusion the amount of antiserum required in the agarose gel is usually about 2% depending on the test arrangement. The amount of beta-thromboglobulin precipitated by 1 ml antiserum is approximately 0.2 mg.

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

Protein Reference(s)

Database Name: UniProt

Accession Number: P02775

Safety Datasheet(s) for this product: