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Rabbit anti Human Calcium Homeostasis Modulator Protein 1

Catalog number: X2723P
Isotype IgG
Product Type Antigen Immunoaffinity Purified Polyclonal
Units 100 µg
Host Rabbit
Species Reactivity Human
Application ELISA
Western Blotting

Background
CALHM1 controls cytosolic Ca2+ permeability and cytosolic Ca2+ concentration. It controls amyloid precursor protein (APP) proteolysis and aggregated amyloid-beta (Abeta) peptides levels in a Ca2+ dependent manner. However, no association between CALHM1 and AlzheimerÕs risk is unclear. Member of the FAM26 family of proteins. CALHM1 is predominantly expressed in the adult brain. It has also been detected in retinoic acid-differentiated SH-SY5Y cells. Also colocalizes with HSPA5 at the endoplasmic reticulum.

Synonyms: CALHM1; FAM26C

Source

Immunogen: Synthetic peptide derived from human CALHM1 protein.

Product

Product Form: Affinity Purified

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Ammonium Sulfate Precipitation

Concentration: See vial for concentration

Applications
Antibody can be used for Western Blotting (1:400 dilution) and immunohistochemistry (2-10 µg/ml). Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: Human hepatocellular carcinoma

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Dreses-Werringloer, U., et la. A Polymorphism in CALHM1 Influences Ca2+ Homeostasis, Ab Levels, and Alzheimer’s Disease Risk. Cell 2008, 133, 1149-1161 2. Bertram, L., et al. 'No association between CALHM1 and Alzheimer’s disease risk.' Cell 2008, 135(6), 993-994. 3. Cui, P.J., et al. 'CALHM1 P86L polymorphism is a risk for Alzheimer’s Disease in the Chinese population.' J. Alzheimers Dis. 2009,

Protein Reference(s)

Database Name: UniProt

Accession Number: Q8IU99

Species Accession: Human

Safety Datasheet(s) for this product:

NM_Sodium Azide

SKU: X2723P Categories : Primary antibodies, Primary antibodies, Antigen Immunoaffinity Purified Polyclonal
Staining on paraffin embedded human hepatocellular carcinoma sections. Primary Ab (immunoaffinity purified) at 10 µg/ml. Antigen retrieval used: 10 mM Na Citrate pH 6.0, 10 minutes pressure cooker method, developed with anti rabbit HRP and DAP substrate. Counterstained with methyl blue. Staining on paraffin embedded human hepatocellular carcinoma sections. Primary Ab (immunoaffinity purified) at 10 µg/ml. Antigen retrieval used: 10 mM Na Citrate pH 6.0, 10 minutes pressure cooker method, developed with anti rabbit HRP and DAP substrate. Counterstained with methyl blue.
Staining on paraffin embedded human hepatocellular carcinoma sections. Primary Ab (immunoaffinity purified) at 10 µg/ml. Antigen retrieval used: 10 mM Na Citrate pH 6.0, 10 minutes pressure cooker method, developed with anti rabbit HRP and DAP substrate. Counterstained with methyl blue.

Staining on paraffin embedded human hepatocellular carcinoma sections. Primary Ab (immunoaffinity purified) at 10 µg/ml. Antigen retrieval used: 10 mM Na Citrate pH 6.0, 10 minutes pressure cooker method, developed with anti rabbit HRP and DAP substrate. Counterstained with methyl blue.