Catalogue

Background
Ubiquitin-like protein which can be covalently attached to target lysines as a monomer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4

Synonyms: Ubiquitin-like protein SMT3C, Sentrin, Ubiquitin-homology domain protein PIC1, Ubiquitin-like protein UBL1. GAP-modifying protein 1, GMP-1

Source

Immunogen: Synthetic peptide derived from the human SUMO-1 protein.

Product

Product Form: Affinity Purified

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Antigen Immunoaffiinity Purification

Concentration: See vial for concentration

Applications
Antibody can be used for Western blotting (1:400 dilution) and immunohistochemistry (10-15µg/ml). Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: Human lung tissue

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Saitoh, H., et al. 'SUMO-1: wrestling with a new ubiquitin-related modifier.' Trends Biochem Sci 1997; 22(10):374-376 2. Muller, S., et al. 'Conjugation with the ubiquitin-related modifier SUMO-1 regulated the partitioning of PML within the nucleus.' EMBO J 1998; 17(1):61-70 3. Navascues J., et al. 'SUMO-1 transiently localizes to Cajal bodies in mammalian neurons.' J Struct Biol 2008; 163(2):137-146.

Protein Reference(s)

Database Name: UniProt

Accession Number: P63165

Species Accession: Human

Safety Datasheet(s) for this product: