Sheep anti Rat ADAR1 (Adenosine Deaminase Acting on RNA 1)
Catalog number: U110P| Isotype | IgG |
| Product Type |
Antigen Immunoaffinity Purified Polyclonal |
| Units | 50 µg |
| Host | Sheep |
| Species Reactivity |
Rat |
| Application |
Western Blotting |
Background
Double stranded RNA specific adenosine deaminase 1 (ADAR1 or DRADA) is an enzyme involved in the editing of mammalian messenger RNAs by the site specific conversion of adenosine to inosine. ADAR 1 is thought to play a role in the editing of transcripts encoding non-NMDA glutamate receptor subunits, the 2C subtype of serotonin receptor, as well as hepatitis delta virus antigen.
Synonyms: DRADA; double strandard RNA specific adenosine deaminase 1, ADAR1
Source
Sheep were immunized with a GST fusion protein containing amino acid 1116-1176 of the rat DRADA coding sequence. The fusion protein was constructed in the pGEX-KG vector by inserting a fragment corresponding to nucleotides +3346 through +3525 of DRADA at the XbaI and XhoI sites in the vector.
Product
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Product Form: Affinity Purified
Purification Method: Antigen Immunoaffiinity Purification
Concentration: See vial for concentration
Applications
Immunoblotting - recommended dilution of 1:1000.
Functional Analysis: Western Blotting
Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Product Stability: See expiration date on vial
Shipping Conditions: Ship at ambient temperature
Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.
References
1. Yang, J. -H., et al. 'Intracellular localization of differentially regulated RNA-specific adenosine deaminase isoforms in inflammation.' J. Biol. Chem. 2003, 278, 45833-45842
2. Wang, Q., et al. 'Stress-induced apoptosis associated with null mutation of ADAR1 RNA editing deaminase gene.' J. Biol. Chem. 2004, 279, 4952-4961
3. Yang, W., et al. 'ADAR1 RNA deaminase limits short interfering RNA efficacy in mammalian cells.' J. Biol. Chem. 2005, 280, 3946-3953
Protein Reference(s)
Database Name: UniProt
Accession Number: P55266 (Rat)
Species Accession: Rat
Safety Datasheet(s) for this product:
| NM_Sodium Azide |