Rabbit anti human Trag 3 (Taxol-resistant-associated protein 3)Catalog number: X1872P
The expression of the cancer-testis antigen Taxol resistance-associated gene-3 (TRAG-3) is associated with acquired paclitaxel (Taxol) resistance, and is expressed in a variety of cancers - e.g., breast cancer, leukemia, and melanoma. Overexpressed in taxol-resistant breast cancer line MDA 435(TR) and the doxorubicin-resistant multiple myelanoma lines 8226/Dox(40) and 8226/MDR(10)V. Weakly expressed in kidney. TRAG-3 is an attractive target for immunotherapy of cancer.First identified as a novel cancer/testis antigen, TRAG-3, (Taxol Resistance Associated Gene-3) was initially discovered in a search for new genes involved in drug resistance. Early studies of TRAG-3 revealed a minimal to absent expression in normal tissues and a marked over-expression in many carcinoma cell lines including several melanoma lines. By RT-PCR evaluation of TRAG-3 two transcripts are seen in many carcinoma cell lines with products in the the 799 bp and a second alternatively spliced transcript.
Synonyms: Chondrosarcoma-associated protein 2, Taxol-resistant-associated protein 3, TRAG-3, CSAG2, TRAG3
Immunogen: Synthetic peptide derived from the human Trag-3 protein.
Product Form: Unconjugated
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
Purification Method: Ammonium Sulfate Precipitation
Concentration: See vial for concentration
Antibody can be used for Western blotting (0.5-2 µg/ml). Optimal concentration should be evaluated by serial dilutions.
Functional Analysis: Western Blotting
Positive Control: Weakly expressed in kidney. Expressed in various tumor cell lines including carcinomas, myeloid and lymphoid malignancies, melanomas and prostate cancer. Overexpressed in taxol-resistant breast cancer line MDA 435(TR) and the doxorubicin-resistant multiple myelanoma lines 8226/Dox(40) and 8226/MDR(10)V.1
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles
Product Stability: See expiration date on vial
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.
1: Janjic B, et al. Spontaneous CD4+ T cell responses against TRAG-3 in patients with melanoma and breast cancers. J Immunol. 2006 Aug 15;177(4):2717-27. 2: Ohta M, et al. Expression of the TRAG-3 gene in human esophageal cancer: the frequent synchronous expression of MAGE-3 gene. Oncol Rep. 2006 Jun;15(6):1529-32. 3:Aung PP, et al. Systematic search for gastric cancer-specific genes based on SAGE data: melanoma inhibitory activity and matrix metalloproteinase-10 are novel prognostic factors in patients with gastric cancer. Oncogene. 2006 Apr 20;25(17):2546-57. 4: Zhu B, et al. In vitro induction of immune response by dendritic cells pulsed with TRAG-3-derived cytotoxic T lymphocyte epitope] Zhonghua Zhong Liu Za Zhi. 2004 Dec;26(12):709-12. Chinese. 5: Meier A, et al. Spontaneous T-cell responses against peptides derived from the Taxol resistance-associated gene-3 (TRAG-3) protein in cancer patients. Cancer Immunol Immunother. 2005 Mar;54(3):219-28. Epub 2004 Oct 2. 6: Yao X, et al. Epigenetic regulation of the taxol resistance-associated gene TRAG-3 in human tumors. Cancer Genet Cytogenet. 2004 May;151(1):1-13. 7: Wu YZ, et al. Expression of TRAG-3 in breast cancer. Int J Cancer. 2003 Oct 20;107(1):167-8.
Database Name: UniProt
Accession Number: Q9Y5P2
Species Accession: Human
Safety Datasheet(s) for this product:
|Western blot of endogenous Trag3 present in Jurkat cell lysate (10 ug/lane) using X1872P (0.5 ug/ml) and developed using anti-rabbit HRP (1:75k) and Pierce’s Super Signal West Femto.|
Western blot of endogenous Trag3 present in Jurkat cell lysate (10 ug/lane) using X1872P (0.5 ug/ml) and developed using anti-rabbit HRP (1:75k) and Pierce’s Super Signal West Femto.