Mouse anti Human CD27Catalog number: 0271
The CD27 antigen recognizes disulfide-linked dimer nerve growth factor (NGF) super family of cells expressing the 55kD surface antigen. CD27 acts as a co-stimulatory on T lymphocytes with its ligand of CD70.
Product Form: Unconjugated
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
Purification Method: Protein A/G Chromatography
Concentration: See vial for concentration
PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8ºC. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20ºC). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Functional Analysis: Flow Cytometry Staining
Product should be stored at 4-8ºC. DO NOT FREEZE
Product Stability: See expiration date on vial
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.
1. Morimoto C., Cluster report : CD27. In Schlossman SF., Boumsell L., Gilks W., et al., eds. Leukocyte Typing V: White Cell Differentiation Antigens. Oxford: Oxford University Press: 1995:356-357. 2. CD27 induction on thymocytes. J Immunol 1990 Sep ;145(5):1356-63 Martorell J; Rojo I; Vilella R; Martinez-Caceres E; Vives J 3. CD27 expression by a distinct subpopulation of human B lymphocytes. Eur J Immunol 1990 De;20(12):2679-84 Maurer D; Holter W; Majdic O; Fischer GF; Knapp W 4. Regulation of expression of CD27, a T cell-specific member of a novel family of membrane receptors. J Immunol 1991 Apr 1;146(8):2488-94 de Jong R; Loenen WA; Brouwer M; van Emmerik L; de Vries EF; Borst J; van Lier RA 5. The T-cell activation antigen CD27 is a member of the growth factor/tumor necrosis factor receptor gene family. Camerini D., Walz G., Loenen W., Borst J., Seed B. J. Immunol. 1991:147(9):3165-3169
Database Name: UniProt
Accession Number: P26842
Safety Datasheet(s) for this product: