Mouse anti Human CD4 FITC - CD8 PECatalog number: 0048
Bi-Testª Reagents (FITC/RPE)
Identification of CD4 on human helper/inducer T cells expressing the 60,000 M.W. surface antigen (HLA class II reactive). CD4 is present in low density on monocytes. Identification of CD8 on human T cells suppressor/cytotoxic expressing the 32,000 M.W. alpha subunit surface antigens.
Synonyms: CD4 FITC - CD8 PE
Immunogen: CD4=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human perherial blood T lymphocytes. CD8=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human perherial blood T lymphocytes.
Product Form: Bi-Test (FITC/RPE) Reagent
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein
Purification Method: Protein A/G Chromatography
Concentration: Titered for flow cytometry
PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2º to 8ºC. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20ºC. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Functional Analysis: Flow Cytometry Staining
Product should be stored at 4-8ºC. DO NOT FREEZE
Product Stability: See expiration date on vial
Shipping Conditions: Room Temperature
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.
1. Thymus Dependent Membrane Antigens in Man: Inhibition of Cell-Mediated Lympholysis by Monoclonal Antibodies to the TH-2 Antigen. Evans, R.L., Wall, D.W., Platsoucas, C.D., Siegal, F.P., Fikrig, S.M., Testa, C.M, and Good, R.A. Proc. Nat. Acad. Sci. 78,544,1981. 2. Novel Immunoregulatory Functions of Phenotypically Distinct Subpopulations of CD4+ cells in the Human Neonate. Clement, L.T., Vink, P.E., Bradley, G.E. J. Immunology 145(1):102-8,1990 . 3. Antigen Presentation by the CD4 Positive Monocyte Subset. Szabo, G., Miller, C.L., Kodys, K., J. Leukoc. Biol. 47(2): 111-20,1990. 4. Human Immunodeficiency Virus Infection is Efficiently Mediated by a Glycolipid-Anchored form of CD4. Diamond, D.C., Finberg, R., Chaudhuri, S., Sleckman, B.P., Burakoff, S.J., Proc. Natl. Acad. Sci. 87(13):5001-5,1990. 5. Development Regulation of the Intrathymic T cell Precursor Population. Adkins, B., J. Immunol. 146(5):1387-93,1991. 6. Induction of CD4 and Susceptibility to HIV-1 Infection in Human CD8+ T Lymphocytes by Human Herpesvirus 6. Lusso, P., De Maria, A., Malnati, M., Lori, F., DeRocco, S.E., Baseler, M., Gallo, R.C., Nat. 349(6309):533-5,1991. 7. Evolutionary Conservation of Surface Molecules that Distinguish T Lymphocyte Helper/Inducer and T Cytotoxic/Suppressor Subpopulations in Mouse and Man. Ledbetter, J.A., Evans, R.L., Lipinski, M., Cunningham-Rundles, C., Good, R.A., and Herzenberg, L.A., J. Exp. Med. 153,310, 1981. 8. Circulating Antigen-Specific Suppressor T Cells in a Healthy Woman: Mechanism of Action and Isolation with a Monoclonal Antibody. Engleman, E.G., Benike, C.J., and Evans, R.L., Clin. Res. 29, 365a 1981. 9. Induction of Immunoglobulin Secreting Cells in the Allogeneic Mixed Leukocyte Reaction: Regulation by Helper and Suppressor Lymphocyte Subsets in Man. Kotzin, B.L., Benike, C.J. and Engleman, E.G., J. Imm. 127,931,1981. 10. CD4 and CD8 molecules can physically associate with the same T-cell receptor. Gallagher, P.F., Fazekas de St. Groth, B., Miller, J.F.A.P., Proc. Nat. Acad. Sci. 1989,86:10044.
Database Name: UniProt
Accession Number: P01730, P01732
Safety Datasheet(s) for this product: