Mouse anti Human IgG IgA IgM IgD IgE (class specific)Catalog number: MAHu/Ig Set
The reactivity of the antibody is restricted to a isotype specific determinant as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining of cytoplasmic immunoglobulins. To identify the presence of immunoglobulin classes in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radioimmunoassay, ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG, subclasses and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly form 1:100 to 1:500; in ELISA from 1:1,000 upwards; in Western blotting 1:2,000 upwards. These data should be interpreted as general recommendations only.
Highly purified monoclonal IgG subclasses isolated from pooled human serum.
Delipidated, heat inactivated, lyophilized stable ascites. Monoclonal antibody concentration in the solution is 1.0 mg/ml. No foreign proteins added. The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C.Reconstitute the lyophilized ascites by adding 0.5 ml sterile distilled water.
Formulation: Delipidated, heat inactivated, lyophilized stable ascites. Monoclonal antibody concentration in the solution is 1.0 mg/ml. No foreign proteins added.
Monoclonal Mouse ascites set containing antibodies to Human IgG, IgA, IgM, IgD and IgE isotype specific.
Species Reactivity: The antibodies do not react with any other component of the Human immunoglobulin system or any other Human plasma protein as tested. These antisera have not been tested for cross-reactivity with other species.
Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation. This will not affect the performance of the product. Diluted ascites should be stored at +4°C, not refrozen, and preferably used the same day. Monoclonal antibodies should not be stored at a temperature below -25ºC due to the aggregation effect of the protein.
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.