Catalogue

Background
The antibody against PAX-5 is well suited for the detection of the protein in tissues and tissue extracts. The cellular localization is nuclear. PAX-5 is a B-cell-specific activator-protein (BSAP). In early phases of B-cell development PAX-5 affects the expression of B-cell specific genes that encode CD-markers like CD19 and CD20. PAX-5 is expressed in pro-, pre- and mature B-cells but not in plasma cells. Interestingly PAX-5 mRNA is found also in the mesencephalon und in the spinal cord during embryogenesis. The expression relocates then to the fetal liver and correlates with the beginning B-cell lymphopoiesis. This shows that PAX-5 is important for the B-cell development and possibly for correct development of the brain. Human PAX-5 protein, 50 kDa..

Source

Immunogen: Synthetic Peptide of a C-terminal sequence of human PAX5 protein

Product
Immunogen-affinity purified rabbit IgG in PBS pH 7.3, 0.5% BSA, 0.09% sodium azide

Purification Method: Immunogen-affinity purified rabbit IgG in PBS pH 7.3, 0.5% BSA, 0.09% sodium azide

Concentration: 40 µg/ml

Specificity

Species Reactivity: Human, Mouse, Rat

Applications
IHC (C,P)

Working Concentration: (liquid conc.) 1:10-1:40

Pre-Treatment: Heat pre-treatment of formalin-fixed tissue with Unmasking Fluid C or G (Art. No. DE000 and DE007)

Positive Control: Tonsil or Raji-cells

Storage
2-8°C

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.

References
1. Kristin C Jensen, John PT Higgins, Kelli Montgomery, Gulsah Kaygusuz, Matt van de Rijn and Yasodha Natkunam The utility of PAX5 immunohistochemistry in the diagnosis of undifferentiated malignant neoplasms Modern Pathology (2007) 20, 871–877 2. Feldman AL, Dogan A.Diagnostic uses of Pax5 immunohistochemistry. Adv Anat Pathol. 2007 Sep;14(5):323-34.

Protein Reference(s)

Database Name: UniProt

Accession Number: Q02548 (PAX5_HUMAN)

Species Accession: Human

Safety Datasheet(s) for this product: