Catalogue

Background
As first published in 1998 by Sakurai et al,, the initial neuropeptides Orexin A and Orexin B were shown to be important in the regulation of appetite. Continued research now suggests that these peptides also influence a wide range of physiological and behavioral processes such as increased EFG arousal and wakefulness and locomotor activity. In addition to the orexins , two G-protein coupled receptors, designated orexin receptor-1 and orexin receptor-2, have been identified which presumably mediate the signaling events from these neuropeptides1. Recently Orexin Receptors have been found to activate extracellular signal-regulated kinases in CHO cells, namely ERK phosporylation, through Ca2+ influx..2. Further studies seem to indicate a mechanism for orexin receptor mediated cell death and suggest a pathway from GPCRÕs to apoptosis throughthe MAPK pathways.3

Synonyms: Ox1r, Hypocretin receptor type 1

Source

Immunogen: Synthetic peptide made to the N terminal of human Orexin Receptor 1

Product

Product Form: Affinity Purified

Formulation: Immunoaffinity antibody povided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Antigen Immunoaffiinity Purification

Concentration: See vial for concentration

Applications
Antibody can be used for Western blotting at Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: ELISA

Positive Control: membrane

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Rodger, R. J. et al, Orexins and Appetite Regularion, Neuropeptides,(2002) Vol 36, pp.303-325. 2.Ammoun, Sylvia et al., OX1 Orexin Receptors Activate Extracellular Signal-Regulated Kinase in Chinese Hamster Ovary Cells via Multiple Mechanisms: The Role of Ca2+ Influx in OX1 Receptor Signaling. Molecular Endocrinology, 2006, Issue 20, vol 1, pp.80-99 3. Ammoun, Sylvia et al. G- protein couples OX1 Orexin/hcrtr-1 Hypocretin Recptors Induce Caspase-dependent and -independent Cell Death through p38 Mitogen-/Stress-activated Protein Kinase, J. Biol. Chem, January 13, 2006, Vol 281, Issue 2, pp834-843,

Protein Reference(s)

Database Name: UniProt

Accession Number: O43613

Species Accession: Human

Safety Datasheet(s) for this product: