Rabbit anti Monkey IgG Fab, conjugated with Horseradish peroxidaseCatalog number: RAMon/Fab/PO
The reactivity of the antiserum is directed to the Fab subunits of intact IgG, IgA IgM and other Ig classes of both light chain types, with their Fab or F(ab’)2 subunits, and with free light chains of kappa and lambda type. In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against pooled monkey serum a characteristic precipitin line is obtained, which shows a reaction of identity with the precipitin line of the purified Fab. Direct staining of fixed cell and tissue substrates; to demonstrate the intracellular presence of free or Ig-bound subunits of both kappa or lambda type. In general this conjugate is not recommended as direct or indirect screening reagent for If isotypes on surface membranes of vital lymphoid cells. The activity to the common Ig/Fab subunit may result in the staining of immunoglobulins bound to the Fc-receptors on non-lymphoid cells. Combinations of isotype-specific reagents should be used instead for this purpose. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Purified normal Fab of polyclonal rhesus monkey IgG. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Horseradish peroxidase-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with he antibody activity. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C.
Product Form: HRP
Horseradish peroxidase–conjugated IgG fraction of polyclonal Rabbit antiSerum to Monkey Fab of IgG.
Species Reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since immunoglobulins of different species frequently share antigenic determinants. Cross-reactivity of this immunoconjugate has not been tested in detail, however in double radial immunodiffusion (Ouchterlony) a reaction has been obtained with Human immunoglobulins.
ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.
The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.